eMALDI in action
In the video above two droplets are evaporated right hand side drop is on a eMALDI plate whereas the left hand side is one a conventional MALDI plate. Notice the difference when the droplets are fully evaporated.
eMALDI is a novel patented technology that transforms the popular analytical detection method MALDI-MS into a more sensitive, robust and faster method by solving a bottleneck in sample preparation stage.
The bottleneck: Despite its popularity, efficiency of MALDI-MS suffers from heterogeneous residue formation during sample preparation step so called coffee stains effect (see the image in blue box and corresponding blue spectra in figure below). The heterogeneous distribution of analyte hampers the efficiency of the MALDI-MS evident from blue spectra in figure.
The solution: eMALDI technology evokes electrowetting to eliminate the undesired heterogeneous patterns to achieve homogeneous distribution of biomolecules of interest for the MALDI-MS (see the red box in figure below). eMALDI not only enhances the detection limits by boosting the signal-to-noise ratio but also makes the detection process faster by eleminating the need for time consuming search for hot spots where the signal is abundant. The signal enhancement is demonstrated in mass spectra with eMALDI in red vs. the spectra with conventional MALDI MS in blue (enlarged in inset for clarity)
The benefits: The patented and unique eMALDI technology provides:
1. faster analysis
2. improved detection sensitivity
3. easy and fault-free analysis
for the popular analytical chemistry technique MALDI-MS.